FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003447984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003447984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10040
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC650.647410358565737Illumina Paired End PCR Primer 2 (97% over 36bp)
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGT280.27888446215139445No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTACTCCATCTCGTA190.1892430278884462Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCGTACTATCTCGTA160.1593625498007968Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGATAGCGATCTCGTA130.1294820717131474Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)