FastQCFastQC Report
Mon 27 Mar 2023
EGAF00003448283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003448283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46048917
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTT5211521.1317356280061917No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA3011980.6540827007940273No Hit
ATCTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG2458160.5338149429225447No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2410970.5235671449124417No Hit
ATGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1496440.32496746883319755No Hit
ATCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTC1193050.2590831832157964No Hit
ATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAA1075090.2334669455961364No Hit
ATCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGA753450.16361948316830122No Hit
ATCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGG651000.1413714029365772No Hit
ATGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAAT643360.1397122976855243No Hit
ATACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAAT603220.13099548030630123No Hit
ATCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA580840.1261354311546567No Hit
ATGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCT572330.12428739637894198No Hit
ATGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAG546290.1186325402614789No Hit
ATAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTA521420.11323176178062992No Hit
ATGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC509430.11062800890626809No Hit
ATGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGA484200.10514905269107633No Hit
ATCACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTG481640.1045931221357497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)