FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003448366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003448366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335672559
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4798190.1429425751778536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC3723040.11091284944742831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7864650.061.0854341
AGCAGTG8048400.059.6048472
GTGGTAT9137500.052.3246276
GTATCAA9495450.050.216389
CAGTGGT9925250.048.3301474
GGTATCA10003400.047.9581538
TGGTATC9966150.047.869717
GCAGTGG11711900.041.316453
AGTGGTA12759450.037.412425
CAACGCA8668800.030.68028512-13
ATCAACG8729600.030.46796410-11
CGCAGAT1856800.028.22576992
TACATGG8977350.028.09069622-23
TCAACGC8582800.028.03848312-13
AGAGTAC9523000.027.26225518-19
GCAGAGT9741350.026.86188916-17
GAGTACA8875000.026.30933220-21
TATCAAC9173800.026.13719410-11
AGTACAT9930000.025.94681720-21
GTACATG8971700.025.76578722-23
Produced by FastQC (version 0.11.5)