FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003448378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003448378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361114660
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6318730.1749784957497987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC5048680.1398082260077727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT9884650.066.52611
AGCAGTG10355950.063.2715842
GTGGTAT12182900.053.2637256
GTATCAA12762450.050.8671469
CAGTGGT12907750.050.377784
TGGTATC13013500.049.6039777
GGTATCA13118550.049.4858478
GCAGTGG15149900.043.356833
AGTGGTA15230450.042.637965
CAACGCA11841550.030.67064712-13
ATCAACG11957800.030.42985510-11
CGCAGAT2355650.029.99978692
TACATGG12085300.028.48036822-23
TCAACGC11734050.027.84257312-13
AGAGTAC12957500.027.38330318-19
GCAGAGT13046050.027.3427216-17
AGTACAT12929600.027.26428420-21
GAGTACA12060850.026.38679320-21
CGCAGAG12551300.026.04696716-17
GTACATG12100400.025.97346522-23
Produced by FastQC (version 0.11.5)