FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003448386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003448386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334142058
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5941030.17779952740938706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4789420.1433348447264307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT10017150.068.100511
AGCAGTG10640650.063.7497022
GTGGTAT13020350.051.6443756
CAGTGGT13531100.049.7731484
GTATCAA13712500.049.198589
GGTATCA13865800.048.6836248
TGGTATC13798150.048.550367
AGTGGTA15042000.044.6785745
GCAGTGG15428050.043.983663
CAACGCA12872300.029.7262312-13
ATCAACG12956650.029.58263210-11
CGCAGAT2345300.028.69188792
CGTATGC216300.028.644061
TACATGG12812200.027.89862622-23
AGAGTAC13798250.027.11847518-19
GCAGAGT13872400.027.07045716-17
AGTACAT13783050.026.904920-21
TCAACGC12756000.026.65683612-13
GAGTACA13063600.025.34487220-21
TATCAAC13484450.025.25954610-11
Produced by FastQC (version 0.11.5)