FastQCFastQC Report
Tue 19 Apr 2022
EGAF00003448388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003448388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347772114
Sequences flagged as poor quality0
Sequence length98
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5420090.15585177137002998No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4458370.12819803027680363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT11599350.069.438311
AGCAGTG12243300.065.627912
GTGGTAT15378200.051.944496
CAGTGGT15840600.050.5855374
GTATCAA16082300.049.6709029
GGTATCA16411600.048.748648
TGGTATC16465050.048.389637
GCAGTGG17474600.046.185383
AGTGGTA17429250.045.780255
CAACGCA15217800.029.75727812-13
ATCAACG15344700.029.49005310-11
TACATGG15407450.028.2175922-23
CGCAGAT2170650.028.08310192
GCAGAGT16255700.027.47574616-17
AGAGTAC16222300.027.36173818-19
AGTACAT16427250.026.88628220-21
TCAACGC15123800.026.69014712-13
GAGTACA15409950.025.5876320-21
TATCAAC15708800.025.57689910-11
GTACATG15514200.025.2366722-23
Produced by FastQC (version 0.11.5)