FastQCFastQC Report
Sat 23 Apr 2022
EGAF00003470731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003470731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27276302
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA1014100.010.524796140-144
GTATCAT963450.010.398049140-144
CCGTATC1111450.010.309046140-144
GCCGTAT1193200.010.183225140-144
CGCCGTA1291550.010.037719140-144
TATCATT942850.09.843269140-144
ATCATTA891150.09.761511140-144
TCATTAA840400.09.694735140-144
TCGCCGT1410900.09.685655140-144
GTCGCCG1530250.09.579535140-144
GGTCGCC1659000.09.381191140-144
CATTAAA814650.09.292357140-144
TGGTCGC1793350.09.233238140-144
GTGGTCG1942700.08.974077140-144
GGTGGTC2181050.08.479298140-144
CGGTGGT2314550.08.354179140-144
TCGGTGG2422000.08.320661140-144
CTCGGTG2578800.08.125202140-144
TCTCGGT2719500.08.051122140-144
AGATCTC3377400.07.3287034140-144
Produced by FastQC (version 0.11.5)