FastQCFastQC Report
Tue 26 Sep 2023
EGAF00003470824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003470824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31371936
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG1205030.3841108180253842No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT978240.31182009296461655No Hit
CCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCC887960.283042780655934No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA884980.28209288709501384No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC826430.26342970991653175No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG789610.2516931055832831No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG775610.24723051838432925No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT769940.2454231705687529No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG754900.24062907689216248No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG750390.23919148630164233No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT714980.22790432825057402No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG697150.22222090469647776No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA669230.21332123079684978No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG656710.20933040281607102No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC641320.20442474445950673No Hit
GTTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATG632450.20159737671274097No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG629130.20053910603413191No Hit
AAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGGC625360.19933739505269932No Hit
GTTTATTATAATAATGTTTGTGTATTCGGCTATGAAGAATAGGGCGAAGG608850.19407472972021875No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT579870.18483717421838422No Hit
GTTTGGATGAGAATGGCTGTTACTACGAGGGCTATGTGGCTGATTGAAGA578980.18455348117502215No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA578320.18434310206421434No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG577580.18410722245512678No Hit
GGCAGGTTAGTTGTTTGTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAAT560810.17876168050323704No Hit
GTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACT540580.17231324200074868No Hit
CTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAATAAGAAG528560.1684817921342183No Hit
GGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCT501180.15975424659797854No Hit
AGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTATGC492880.15710856990145589No Hit
GGGGATTATTGCTAGGATGAGGATGGATAGTAATAGGGCAAGGACGCCTC485510.15475933649743517No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC483740.154195137972996No Hit
GGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCG482020.15364687725998166No Hit
GGTGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGG473650.15097888762746425No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT472540.1506250682138329No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG470770.15006086968939375No Hit
GTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGA459690.14652905067765024No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC452730.14431050732731318No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC438940.13991485893634362No Hit
GTAAATATGTAGAGGGAGTATAGGGCTGTGACTAGTATGTTGAGTCCTGT438290.13970766738782076No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG423370.13495182445864992No Hit
GTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATAAGAACAGGGAGG422990.1348306970918212No Hit
GGAAGTTTTTTCATAGGAGGTGTATGAGTTGGTCGTAGCGGAATCGGGGG414910.1322551467655678No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA411900.13129569051779272No Hit
GGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTA407960.1300397909775157No Hit
GTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGG406530.1295839695707654No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT406460.12956165663477065No Hit
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT399280.1272729869141643No Hit
GGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGT391340.12474206245990047No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA390210.1243818679216992No Hit
GTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAATGGTT388860.12395154701322865No Hit
CTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGATA376840.12012009714669825No Hit
CTAGGAGTCAATAAAGTGATTGGCTTAGTGGGCGAAATATTATGCTTTGT376070.11987465485075578No Hit
GGGAAGGTTAATGGTTGATATTGCTAGGGTGGTGCTTCCAATTAGGTGCA371910.11854862894020948No Hit
GGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAG367450.1171269761611142No Hit
GAAGTTTTTTCATAGGAGGTGTATGAGTTGGTCGTAGCGGAATCGGGGGT364040.11604001742194044No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA363940.11600814179909076No Hit
CTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG363150.11575632437857836No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT362560.1155682582037653No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC360310.11485105668964772No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT357000.11379597357332363No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT355180.11321583723745964No Hit
GGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAATAAGA353960.11282695463869365No Hit
GTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTG352240.11227869392567932No Hit
GTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTCTTCGA348740.11116304712594084No Hit
GGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGATAATGGCCCATTT338640.10794360921812411No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT337720.10765035348790715No Hit
GTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTGTTAGATAT335190.10684390022981048No Hit
CCGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGA333660.10635620320021053No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA333150.1061936375236772No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA332610.10602150916028899No Hit
GTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGG330390.1053138703330263No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG328290.10464448225318322No Hit
GTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATCTTA327290.10432572602468652No Hit
CTGATGGTTTCGATAATAACTAGTATGGGGATAAGGGGTGTAGGTGTGCC322270.10272556975763306No Hit
GTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGC321360.10243550158970108No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT320820.10226337322631283No Hit
AGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGGCG320060.10202111849265535No Hit
GTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGA319680.1018999911258266No Hit
GCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAAATAATAAGAA317110.10108078761859006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)