FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003470881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003470881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95121
Sequences flagged as poor quality0
Sequence length25-270
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT4540.4772868241502927No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA4300.45205580260930817No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG3160.3322084502896311No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT2340.24600246002460024No Hit
GTTTTTTGGAAAGTCATGTCAGTGGTAGTAATATAATTGTTGGGACGATT2060.21656626822678482No Hit
CATTTTCTACCCCAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCG1860.19554041694263097No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1860.19554041694263097No Hit
CATAAAGCCATTTACCGTACATAGCACATTACAGTCAAATCCTTTCTCGC1790.1881813689931771No Hit
CATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACAC1740.18292490617213863No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1280.13456544821858474No Hit
CATTACAGTCAAATCCTTTCTCGCCCCCATGGATGACCCCCCTCAGATAG1180.12405252257650783No Hit
CATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCCTCCTGCTTGCA990.10407796385656164No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT960.10092408616393857No Hit
CTCAATCTCCAACACATATGGCCTAGACTACGTACATAACCTAAACCTAC960.10092408616393857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)