FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003471023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644
Sequences flagged as poor quality0
Sequence length94
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.30413625304136255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.24330900243309003No Hit
AGTCTGGAGAGCTGCATGGGCTCACAACTGAGGAGGAATTTGTAGAAGGG30.18248175182481752No Hit
GGGGTAGGAAATCCTCCAGGATCTTAGGAAATTTCACTCCGCTTCTCCTG30.18248175182481752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.18248175182481752No Hit
GGACGTCATAGAGGGTGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAA20.12165450121654502RNA PCR Primer, Index 26 (96% over 29bp)
GTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATAT20.12165450121654502No Hit
CTACAGGAGGAAACGCGAGCGTGGTGGGACTGGCTCCAGGCACACAGGCG20.12165450121654502No Hit
GGGTCATATATCTTGGTGCATCCATTAAGTTCATTGTAACATTTGGCCTC20.12165450121654502No Hit
GGGTGTTCTTCTCCTGCGTCTCCGTTTTCTTCAGCTTGGCCTTATCGAAG20.12165450121654502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC20.12165450121654502No Hit
ACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAG20.12165450121654502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAAA20.12165450121654502No Hit
TACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTTA20.12165450121654502No Hit
CTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATT20.12165450121654502No Hit
GAATGGCTGTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATG20.12165450121654502No Hit
CTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGCG20.12165450121654502No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGTAT20.12165450121654502No Hit
GGATGCTTCTGAGTGGCCACCGACCTAAGTTCCTTGGAACAGGACGTCAT20.12165450121654502No Hit
GCTGGAGACAATGACAGGGGAGAAAGTCAAGACAGTGGTTCAGTTGGAAG20.12165450121654502No Hit
TCTCTACACTGGTCCAGGTCCTTCATTATTTTGTCCACAGCCAGAGGGTC20.12165450121654502No Hit
AATTTGTAGAGGATGCTTCTGAGTGGCCACCGACCTAAGTTCCTTGGAAC20.12165450121654502No Hit
GTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTTTTTACAA20.12165450121654502No Hit
ATCCCATCCCTCGTCCTTCAGGTCCACTGGAGGAGAAGTCCCTCATTCCT20.12165450121654502No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA20.12165450121654502No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGT20.12165450121654502No Hit
GAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGA20.12165450121654502No Hit
TTGTAGAGGATGCTTCTGAGTGGCCACCGACCTAAGTTCCTTGGAACAGG20.12165450121654502No Hit
ATTGAAGAGTATGCAATGAGCGATTTTAGGTCTGTTTGTCGTATGCCGTC20.12165450121654502No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC20.12165450121654502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.12165450121654502No Hit
GTATATCCCTTCTACAAATTCCTCCTCAGTTGTGAGCCCATGCAGCTCTC20.12165450121654502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)