FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003471032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2176017
Sequences flagged as poor quality0
Sequence length94
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGAC68570.315117023442372No Hit
TGTGTGGAGAGCCTGGTTTCTCAGTACTTCCAGACCGTGACTGACTATGG45070.2071215436276463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39760.18271916074185082No Hit
GTCTGGAAGTACTGAGAAACCAGGCTCTCCACACATGGCTCCTTTGCCTG32280.14834442929444025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.13584452694992732No Hit
GTGCTACTCCTCACCATCTGCAGCCTTGAAGGAGCTTTGGTTCGGAGACA29440.13529306067002234No Hit
CTTCCAGACCGTGACTGACTATGGCAAGGACCTGATGGAGAAGGTCAAGA29360.13492541648341902No Hit
CTCCACACATGGCTCCTTTGCCTGTCTCCGAACCAAAGCTCCTTCAAGGC27290.12541262315505808No Hit
GGTCTGGACACTTCACTGGGTGGCAGGCTGTGTTCCAAGTTCCACGAAAT22800.10477859318194666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.1033999274821842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)