FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003471047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139458
Sequences flagged as poor quality0
Sequence length94
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8080.5793859083021411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.3915157251645656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.3276972278392061No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT3890.27893702763556055RNA PCR Primer, Index 26 (100% over 22bp)
CCGCTACACCATTGCCGCCCTGCTGAGCCCCTACTCCTATTCCACCACGG3030.2172697156133029No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT2170.15560240359104532RNA PCR Primer, Index 26 (100% over 24bp)
GTGTACGTGGATGTGCTCAAAGACAGCGGCAGAGACTATGTGTCCCAGTT1970.14126116823703194No Hit
GTACGGACATTCAGACTGCTGCAGCCAAAGTGAAGAGGGAAGACATAACT1940.13910998293392993No Hit
ATCCAGAACACTGCATAGAAATGAATATGGAATAGCTTCCATATTGGATT1870.13409055056002525No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT1700.12190050050911384No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)