FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003471079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4587
Sequences flagged as poor quality0
Sequence length94
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT2375.166775670372792No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT1302.8340963592762156RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT681.4824504033137127RNA PCR Primer, Index 23 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561.2208415086112927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.9810333551340745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.9156311314584695No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC380.8284281665576629No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG270.5886200130804448RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT200.4360148245040331RNA PCR Primer, Index 23 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT140.3052103771528232RNA PCR Primer, Index 23 (95% over 22bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG110.2398081534772182Illumina PCR Primer Index 1 (95% over 23bp)
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA90.1962066710268149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA80.17440592980161324No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1526051885764116No Hit
GTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA70.1526051885764116No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA70.1526051885764116No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1526051885764116No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC70.1526051885764116Illumina PCR Primer Index 1 (95% over 24bp)
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA60.13080444735120994No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA60.13080444735120994RNA PCR Primer, Index 23 (96% over 30bp)
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA50.10900370612600828No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTTGTATCTGTCTCTTATACACA50.10900370612600828No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA50.10900370612600828No Hit
TATCAACGCAGAGTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAAA50.10900370612600828No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAA50.10900370612600828No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10900370612600828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG50.10900370612600828No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)