FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003471175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362377
Sequences flagged as poor quality0
Sequence length89
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT40351.1134812639875047No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT18650.5146573871962072RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.35736263614964525No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT10630.29334091291665865RNA PCR Primer, Index 23 (96% over 25bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG8110.22380007561186277RNA PCR Primer, Index 23 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.21496949309696808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.1785433402230274No Hit
CTCTAGGACTGGCCAGTGGGTGTTCTAGAGGCCAGCTGGGGTTGGAAGAC5270.14542865579217223No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4490.1239041109121164No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT4420.12197242098698317RNA PCR Primer, Index 23 (96% over 28bp)
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4170.11507352839722168No Hit
ATCCAGAACACTGCATAGAAATGAATATGGAATAGCTTCCATATTGGATT3840.10596699017873651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)