FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003471185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614109
Sequences flagged as poor quality0
Sequence length89
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24050.3916242881963951No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23410.3812026855167405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.36052231769930093No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT12860.2094090788443094TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT8120.13222408399811758No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC7660.12473355707211588No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT7610.12391936936276784RNA PCR Primer, Index 1 (95% over 23bp)
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG6560.10682142746645953No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)