FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003471187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15951
Sequences flagged as poor quality0
Sequence length89
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT2531.5861074540781142No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT1310.8212651244436086RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.5893047457839634No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT880.5516895492445615RNA PCR Primer, Index 31 (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG500.31345997116168267RNA PCR Primer, Index 31 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.3009215723152154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.23822957808287884No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT360.22569117923641152RNA PCR Primer, Index 31 (96% over 27bp)
GTATAAAATCAGGCAGTTTTTGATCACGTTTATCGTATGCCGTCTTCTGC300.1880759826970096TruSeq Adapter, Index 27 (95% over 22bp)
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC230.14419158673437402No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT200.12538398846467305RNA PCR Primer, Index 31 (95% over 21bp)
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA200.12538398846467305No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT190.11911478904143942No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC190.11911478904143942No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA160.10030719077173844No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC160.10030719077173844No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)