FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003471189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149232
Sequences flagged as poor quality0
Sequence length89
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT25171.6866355741395949No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT17531.1746810335584863RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT10930.7324166398627641RNA PCR Primer, Index 33 (95% over 24bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG8220.5508201994210358RNA PCR Primer, Index 33 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.4945320038597619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.32030663664629566No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT3930.26334834351881636RNA PCR Primer, Index 33 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3480.23319395303956258No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2880.1929880990672242No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC2760.18494692827275652No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2700.18092634287552267No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA2350.1574729280583253No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT2230.1494317572638576RNA PCR Primer, Index 33 (95% over 21bp)
GTTTAAGCATATCACTAAGCGGAGGAGAAGAAACTAACCAGGATTTCCCT1970.132009220542511No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1920.1286587327114828No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1800.12061756191701511No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG1780.11927736678460382RNA PCR Primer, Index 15 (95% over 24bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA1740.11659697651978129RNA PCR Primer, Index 33 (96% over 29bp)
TTCCCAAACAGCCCGACTCGACGACCACACCTCGTGGAGCAACACGGTCC1580.10587541546049105No Hit
AGTCTGGACCGTGTTGCTCCACGAGGTGTGGTCGTCGAGTCGGGCTGTTT1500.10051463493084593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)