FastQCFastQC Report
Fri 2 Sep 2022
EGAF00003471320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294118
Sequences flagged as poor quality0
Sequence length89
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71542.4323570811715025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41701.4177982986420417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40031.3610183667779598No Hit
GTATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCC14420.490279411664706No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13600.46239944512066583No Hit
GTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCTTGTGCAAAAAA13450.4572994512406585No Hit
TATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCC11200.38079954304054836No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA8630.2934196478964225No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA8010.27233967319239216No Hit
GGTATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCC7750.26349968380037947No Hit
ACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCTTGTG7750.26349968380037947No Hit
GCACAAGGGGCCTCGATATCCAGGCTCCGTAGTCGCCCATGTACTCTGCG7610.2587396895123726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7520.2556796931843682No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7220.2454797054243535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6070.20637975234429717No Hit
GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5860.19923976091228693No Hit
GTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCTTGTGCAAATCC5450.18529977764026684No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA5300.1801997837602595No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.17407979110425068No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5010.1703397955922453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4920.16727979926424089No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4850.16489980212023744No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.16081980701623158No Hit
GTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4680.15911980905622913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4550.15469981436022276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4520.1536798155842213No Hit
GTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.15333981599222082No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.14279982864020563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3930.13361983965619242No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA3900.13259984088019094No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT3890.13225984128819046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3810.12953984455218653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3670.12477985026417968No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA3340.11355986372816353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3060.10403987515214981No Hit
ATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCT3050.10369987556014931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT3050.10369987556014931No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.10369987556014931No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3020.10267987678414786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3000.10199987760014688No Hit
CTGCATACCGAGACTAAACATGGATTTGCACAAGGGGCCTCGATATCCAG3000.10199987760014688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2990.1016598780081464No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA2970.10097987882414543No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)