FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003471409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736173
Sequences flagged as poor quality0
Sequence length89
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314201.8097274868345492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221131.2736633964472435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153230.8825733380256461No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC53200.30642107670145774No Hit
GTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCTTGTGCAAAAAA52320.3013524573875991No Hit
TATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCC46370.2670816790723044No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC45680.2631074207466652No Hit
GTATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCC45100.2597667398352584No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA44410.25579248150961914No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA43190.24876553200631504No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA39210.2258415492004541No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.20775579392145826No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC34420.19825213270797323No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC29740.1712962936297247No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC28250.16271419956421393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27360.15758798230360685No Hit
ACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCTTGTG26150.15061863074705115No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26110.15038823896005754No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA25460.14664437242141193No Hit
GGTATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCC24440.14076938185307572No Hit
GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA23800.13708311326117847No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.13535517485872664No Hit
GCACAAGGGGCCTCGATATCCAGGCTCCGTAGTCGCCCATGTACTCTGCG23320.13431841181725554No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC22340.1286738130359129No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT21540.12406597729604134No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA20730.1194005436094214No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG20200.11634785243175652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC19640.11312236741384642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG19550.11260398589311088No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT19520.1124311920528657No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA19090.10995448034268475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC19080.10989688239593635No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA18870.10868732551422007No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC18790.1082265419402329No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA17950.10338831441336778No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)