FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003471531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063591
Sequences flagged as poor quality0
Sequence length101
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239452.2513353347292333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135711.2759604020718491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118801.1169707152467443No Hit
GTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCTTGTGCAAAAAA35530.3340569824302763No Hit
GTATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCC33800.3177913314422555No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC32920.30951747429227966No Hit
TATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCC32170.3024658914940047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG27330.25695967716913737No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA25020.23524080215045068No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC23950.22518054402491183No Hit
GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA22740.21380399044369497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC22510.21164150505222404No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA22330.20994912518063807No Hit
GCACAAGGGGCCTCGATATCCAGGCTCCGTAGTCGCCCATGTACTCTGCG20270.1905807777613763No Hit
GTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20230.19020469334546833No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC19420.18258898392333145No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA19210.18061454073981445No Hit
GGTATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCC18850.1772297809966425No Hit
ACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCTTGTG18630.17516131670914853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC17450.1640668264398627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT17340.1630325942961157No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT17060.16040000338475974No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA15290.143758267980831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15240.14328816246094597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG15070.141689803693337No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA15050.14150176148538302No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14830.13943329719788905No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC14800.13915123388595804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC14770.13886917057402706No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC14410.1354844108308551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT14410.1354844108308551No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14010.13172356667177515No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT13620.1280567436166722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG13500.1269284903689482No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG13440.12636436374508622No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT12440.11696225334738636No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA12390.11649214782750135No Hit
GTATCAACGCAGAGTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAA12360.11621008451557036No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12290.11555193678773137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12010.11291934587637541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT11590.10897045950934146No Hit
CTGCATACCGAGACTAAACATGGATTTGCACAAGGGGCCTCGATATCCAG11560.10868839619741046No Hit
GTATCAACGCAGAGTACATGGGGTACTCTAGATGGACTACAAAGACGATG11350.10671395301389351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT11310.1063378685979855No Hit
GTACATGGGACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA11140.10473950983037653No Hit
TATCAACGCAGAGTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAAA10830.10182485560708958No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA10770.10126072898322758No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC10720.10079062346334258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT10660.10022649683948058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)