FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003471547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763720
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288643.779395590006809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161752.1179227989315454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155572.0370030901377465No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA42060.5507253967422615No Hit
GTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38300.5014926936573614No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33320.4362855496778924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG26640.3488189388781229No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC26010.3405698423505997No Hit
GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA24790.324595401456031No Hit
GTACATGGGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA23630.3094065888021788No Hit
GTATCAACGCAGAGTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAA22120.2896349447441471No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA21930.2871471219818782No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA21420.28046928193578796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT21000.27496988425077257No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC20800.2723511234483842No Hit
TATCAACGCAGAGTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAAA20690.27091080500707065No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.26750641596396585No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA19950.2612213900382339No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19470.254936364112502No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA18220.23856910909757506No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA18140.2375216047766197No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA17960.2351647200544702No Hit
GTACATGGGACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA16930.2216781019221704No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC16490.21591682815691615No Hit
GTACATGGGACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA16000.20950086419106476No Hit
GGTATCAACGCAGAGTACATGGGATGGTATCAACGCAAAAAAAAAAAAAA15930.2085842979102289No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA15900.20819148378987065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT15830.2072749175090347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15120.19797831666055624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT14760.19326454721625727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT14510.1899910962132719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG14340.18776514953124182No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA14070.1842298224480176No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT13410.1755879118001362No Hit
ACGCAGAGTACATGGGATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA13360.1749332215995391No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA13040.1707432043157178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT12740.16681506311213534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT12740.16681506311213534No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA12640.16550568271094118No Hit
GTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCCTTGTGCAAAAAA12580.1647200544702247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC12440.1628869219085529No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC12330.1614466034672393No Hit
GTATCAACGCAGAGTACATGGGCATGGTATCAACGCAAAAAAAAAAAAAA12290.16092285130676165No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC11980.15686377206305976No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA11960.15660189598282093No Hit
GTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAA11550.15123343633792488No Hit
TATCAACGCAGAGTACATGGGCATGGTATCAACGCAAAAAAAAAAAAAAA11540.15110249829780548No Hit
GTATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCC11430.1496621798564919No Hit
GGTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAA11150.14599591473314827No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11140.14586497669302886No Hit
GTACATGGGCTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11030.1444246582517153No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT10870.14232964960980465No Hit
TATCAACGCAGAGTACATGGGCGACTACGGAGCCTGGATATCGAGGCCCC10690.13997276488765517No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG10240.13408055308228145No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA10220.13381867700204264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10110.13237835856072905No Hit
TATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAA10090.1321164824804902No Hit
GTATCAACGCAGAGTACATGGGGTACTCTAGATGGACTACAAAGACGATG9870.12923584559786308No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA9700.12700989891583303No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT9530.12478395223380295No Hit
GTATCAACGCAGAGTACATGGGACTGGTATCAACGCAAAAAAAAAAAAAA9520.12465301419368356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA9270.12137956319069815No Hit
TATCAACGCAGAGTACATGGGGTACTCTAGATGGACTACAAAGACGATGA9100.11915361650866811No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA9040.1183679882679516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT8940.11705860786675745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC8640.11313046666317499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT8390.1098570156601896No Hit
TATCAACGCAGAGTACATGGGACTGGTATCAACGCAAAAAAAAAAAAAAA8370.10959513957995078No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC8350.10933326349971194No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC8280.10841669721887602No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA8150.10671450269732362No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA8150.10671450269732362No Hit
ACGCAGAGTACATGGGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7970.10435761797517415No Hit
GTATCAACGCAGAGTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAA7890.10331011365421881No Hit
GGTATCAACGCAGAGTACATGGGCATGGTATCAACGCAAAAAAAAAAAAA7870.10304823757397999No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)