FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36136794
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG1141480.3158774959394572No Hit
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC986530.27299876131789663Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG709860.19643690583066115No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC637460.1764019243101643TruSeq Adapter, Index 1 (100% over 51bp)
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG615430.17030564471214574No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC615250.1702558339846086No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA547680.1515574403196919No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACAC516720.14298999518330266No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG489200.1353744883954011No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG402500.11138232129834208No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCGCGTATGCC384000.10626288541257976TruSeq Adapter, Index 1 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)