FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36136794
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1200850.33230673423879276No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1033510.285999361205092Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG932910.2581606990371088No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA874740.24206353225468755No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG639670.17701348935381483No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG608850.16848478589439891No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG500960.13862878926116134No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG441200.12209162771882863No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG432340.11963983301894462No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA386830.10704602073996934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)