FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39226848
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1807070.460671731769017Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1802830.4595908394169218TruSeq Adapter, Index 2 (100% over 51bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG1276340.32537409072480156No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC906290.23103819098592884No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG885090.22563372922545294No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG877440.22368353429773405No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGCC828370.2111742447417646TruSeq Adapter, Index 2 (98% over 51bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG795680.2028406666780874No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACAC690750.1760911302381471No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG633250.1614328023500639No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA613320.15635209844033351No Hit
CGGGAGGTGAGGGGCGTTTTTGTTTGAGATCGGAAGAGCACACGTCTGAAC597860.15241092019425065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG518260.13211869584831287No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG436300.11122484274035987TruSeq Adapter, Index 2 (100% over 51bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGT403990.10298813710446478No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG395240.1007575219910608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)