FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39226848
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1722430.4390946731177585Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1458900.37191364445086184No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1423320.3628433260811575No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA1043660.2660575736291634No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG901510.22981963781540643No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG894460.22802239935260665No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG880610.22449165428739012No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG683970.17436272218456095No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG630580.1607521460811738No Hit
CAAACAAAAACGCCCCTCACCTCCCGAGATCGGAAGAGCGTCGTGTAGGGA580760.14805166094405545Illumina Single End PCR Primer 1 (100% over 25bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA513310.13085680501273006No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG509820.12996710824178379No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA406480.10362290643387917No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTC396310.10103029435349993No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAAT393280.10025786420565833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)