FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37032440
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1395090.37672105861779565Illumina Single End PCR Primer 1 (100% over 32bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1320240.356509049903274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1025750.27698687961149737No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA954210.2576686818367896No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG884050.2387231303149347No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG845610.2283430419383654No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG829630.22402790634373537No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG677990.18308002389256553No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG547250.1477758419375013No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG475560.12841713913530947No Hit
CAAACAAAAACGCCCCTCACCTCCCGAGATCGGAAGAGCGTCGTGTAGGGA462980.12502011749698372Illumina Single End PCR Primer 1 (100% over 25bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA462810.12497421179916851No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)