FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34073085
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3298720.9681307107941649TruSeq Adapter, Index 6 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCC2183520.640834253781247TruSeq Adapter, Index 6 (98% over 51bp)
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1249230.3666324901311402Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG1119470.32854964556335303No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG676170.19844695600647844No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCCG641320.18821894172482476TruSeq Adapter, Index 6 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG631850.1854396219185906TruSeq Adapter, Index 6 (100% over 51bp)
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG624730.18334999604526564No Hit
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCA605220.17762406896821933Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG602020.17668491127234295No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC587000.1722767398373232No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACAC489800.1437498248250782No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA466690.136967345340171No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG450970.13235373315917828No Hit
CGGGAGGTGAGGGGCGTTTTTGTTTGAGATCGGAAGAGCACACGTCTGAAC430010.126202250251188Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG393260.1154166110876077No Hit
GAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGCTC353600.10377692539433984Illumina Single End PCR Primer 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)