FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34073085
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2773930.8141117835382384No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1215620.3567683994566386No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1170750.34359964763977197Illumina Single End PCR Primer 1 (100% over 32bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA864720.25378388836819443No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG640080.18785501811767266No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG606420.177976253104173No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG586710.1721916286711344No Hit
GGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGA517680.15193223625040117Illumina Single End PCR Primer 1 (100% over 29bp)
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG489200.1435737327571014No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG451680.13256210877295085No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA441090.12945408377315998No Hit
CAAACAAAAACGCCCCTCACCTCCCGAGATCGGAAGAGCGTCGTGTAGGGA416210.12215213268772113Illumina Single End PCR Primer 1 (100% over 25bp)
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG378800.11117279224936634No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA358290.10515337839235865No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)