FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32161262
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC6802292.115056927803393TruSeq Adapter, Index 7 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCC5177571.6098777467128TruSeq Adapter, Index 7 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCCG1714640.5331382829442451TruSeq Adapter, Index 7 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG1531440.476175344114295TruSeq Adapter, Index 7 (100% over 51bp)
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1045750.3251582602697618Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG983230.3057187245948247No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG614750.19114610614471536No Hit
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCA587700.18273536654127565Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTT578800.1799680621985543TruSeq Adapter, Index 7 (100% over 51bp)
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG508100.1579850939928912No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG502430.15622210347342713No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC499450.1552955229182238No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG496500.15437827035518695No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA472210.14682570603106307No Hit
GAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGCTC435080.13528076105968728Illumina Single End PCR Primer 1 (96% over 28bp)
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG410100.12751365291573447No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACAC390010.12126700749491733No Hit
CGGGAGGTGAGGGGCGTTTTTGTTTGAGATCGGAAGAGCACACGTCTGAAC365780.11373309915512644Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)