FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32161262
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6451372.0059442941013943No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1125760.35003601537775475No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG988280.3072889366095149Illumina Single End PCR Primer 1 (100% over 32bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA810200.2519179751093101No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG529450.16462351508470036No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG513500.15966413258285697No Hit
GGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGA506600.15751869438456736Illumina Single End PCR Primer 1 (100% over 29bp)
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG501480.15592671705482203No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG499220.15522400831161415No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA403290.12539619869394428No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG399340.12416801305869155No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG389860.12122036753408494No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGG366390.11392276832917812No Hit
CAAACAAAAACGCCCCTCACCTCCCGAGATCGGAAGAGCGTCGTGTAGGGA356060.11071082969318805Illumina Single End PCR Primer 1 (100% over 25bp)
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAAT321740.10003960665473886No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)