FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40940909
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2271100.5547263252020125No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2048970.5004700799388699Illumina Single End PCR Primer 1 (100% over 32bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1424090.34784034716962436No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA1041640.254425225390086No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG867320.2118467863036456No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG833720.20363983613553865No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG712140.17394337775939464No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG637620.15574153470798607No Hit
CAAACAAAAACGCCCCTCACCTCCCGAGATCGGAAGAGCGTCGTGTAGGGA628650.15355057211846468Illumina Single End PCR Primer 1 (100% over 25bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA450110.10994137917162514No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG414290.10119218408169686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)