FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37757969
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1453180.3848670991811027Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1363770.36118732975282647No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1286160.3406327284182049No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA949540.25148068742786456No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG698430.18497552132637218No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG685840.18164112587729495No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG588000.1557287151753316No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG470080.12449822181908143No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG464510.12302303654097496No Hit
CAAACAAAAACGCCCCTCACCTCCCGAGATCGGAAGAGCGTCGTGTAGGGA445580.1180095253534426Illumina Single End PCR Primer 1 (100% over 25bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA415390.11001386223925338No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG402590.10662384939189923No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)