FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26404925
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC4365711.6533695891959548TruSeq Adapter, Index 10 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGCC2061510.7807293525734309TruSeq Adapter, Index 10 (98% over 51bp)
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCA843090.31929270770509666Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG840990.3184974015264198No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG687520.2603756685542565TruSeq Adapter, Index 10 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGCCG619950.23478574546225753TruSeq Adapter, Index 10 (98% over 51bp)
GAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGCTC580350.21978854323577895Illumina Single End PCR Primer 1 (96% over 28bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA524160.19850842219775286No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG518300.1962891392420164No Hit
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC482830.1828560391669357Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG442900.16773386025523648No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG430270.16295066166633687No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG379190.14360578566309126No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAA357470.1353800474722045No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC327480.12402231780624258No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG317920.120401781107123No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTA315610.11952694431057842No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG315330.11942090348675484No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGT288870.10940004563542596No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGG283220.10726029329755718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)