FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26404925
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2257550.8549730779390587No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1259190.4768769462515042No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA883790.3347064988823108No Hit
GGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGA795270.30118244986493997Illumina Single End PCR Primer 1 (100% over 29bp)
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG459080.17386150500332798Illumina Single End PCR Primer 1 (100% over 32bp)
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG435910.16508662683192624No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG429050.1624886266482484No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTGCTC399360.15124451215066886Illumina Single End PCR Primer 1 (96% over 30bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA337620.12786251049756817No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG322970.12231430310822697No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG322690.12220826228440339No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG312900.11850062062285728No Hit
CAAACAATAAACCATAACCAATACTACCAATCAATACTCATCATTAATAAT298370.11299785930086906No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG287450.10886226717174922No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTC280940.10639681801785084No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA271420.10279143000784892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)