FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27905023
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC4191681.5021238291041725TruSeq Adapter, Index 11 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGCC3161511.132953733813443TruSeq Adapter, Index 11 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG1090840.3909117007357421TruSeq Adapter, Index 11 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGCCG1088940.39023081973449725TruSeq Adapter, Index 11 (98% over 51bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG898380.3219420388938579No Hit
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC623170.22331821765565288Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG557010.1996092244754645No Hit
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCA500710.17943364533331507Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA437570.15680689458668426No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG385560.13816867307366132No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG335380.12018624747236366No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC333670.11957345457124333No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG327670.11742330404099649No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG327550.11738030103039154No Hit
GAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGCTC311030.11146021990377862Illumina Single End PCR Primer 1 (96% over 28bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG303600.10879761683048964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)