FastQCFastQC Report
Wed 8 May 2024
EGAF00003471675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29014480
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4617851.5915673829067418TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC3256181.1222603334610857TruSeq Adapter, Index 12 (98% over 50bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1104970.3808339835833694No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA670460.23107772395024828No Hit
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGT580900.20021037771485134Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCC563750.19429953595583999TruSeq Adapter, Index 12 (98% over 50bp)
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCC553800.19087021376912494Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC540630.1863311008847996TruSeq Adapter, Index 12 (100% over 50bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT520500.17939318574725446No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTT427660.1473953694844781No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGA404550.13943038096839924No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGA387110.13341958911550372No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTT379430.1307726349050543No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC372290.12831179466252712No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACA371540.12805330304041293No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA335830.1157456552728155No Hit
GAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGCT322800.11125479415795149Illumina Single End PCR Primer 1 (96% over 27bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT313330.10799090660938951No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAG309550.10668810883393395No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTT309250.10658471218508826No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACA301920.10405838739829217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)