FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29014480
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC4611211.5892788704122907TruSeq Adapter, Index 12 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCC3252741.1210747185543217TruSeq Adapter, Index 12 (98% over 51bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG1091960.3763500155784284No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG665750.2294543965633711No Hit
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC580550.20008974829119805Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGCCG563730.19429264284591694TruSeq Adapter, Index 12 (98% over 51bp)
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCA553120.1906358480317414Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG540620.18632765432983808TruSeq Adapter, Index 12 (100% over 51bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA518940.17885552317325693No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG423170.14584786630675442No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG403770.13916154968140046No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG386490.1332059027078893No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG376770.1298558512852893No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG371780.12813602035948946No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC371200.12793612017172115No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATAG331870.11438081950805254No Hit
GAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGCTC317110.10929370438484509Illumina Single End PCR Primer 1 (96% over 28bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTA312010.10753596135446852No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGT309130.10654335352555No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTT306980.10580234420882262No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACAC301470.10390329242502365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)