FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21174003
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG812400.3836780414171095No Hit
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC585840.27667890667626716Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG503820.23794272627617932No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG390840.18458484208205694TruSeq Adapter, Index 13 (97% over 40bp)
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC378240.178634148677508No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG369280.1744025444787176No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA359990.17001508878599858No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG359120.1696042075747321No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG350480.1655237320973271No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACAC294190.13893924545113173No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG292760.13826388897744088No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG267770.1264616803917521No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATAG245870.1161188085219408No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCGCGTATG226440.10694246147032282TruSeq Adapter, Index 13 (97% over 40bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTA223400.10550673861716181No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)