FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21174003
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA921640.43526960868004033No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA654830.3092613144524443No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG549640.2595824700695471Illumina Single End PCR Primer 1 (100% over 32bp)
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG375820.17749123772203113No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG371090.1752573663090536No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG368250.17391609890675844No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG339250.16022005853120924No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA321960.15205438480385594No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG291790.13780578004074145No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG291610.1377207701349622No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA259290.12245676927503978No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG252640.11931612553375004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231830.10948803587115766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)