FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24280509
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG2141250.8818801945214574TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCGCGTATG1394170.5741930698405046TruSeq Adapter, Index 7 (97% over 36bp)
TGGATGTTTAAATTAAATTATTTTTATTGTTATATGATTGAGATCGGAAGA1263680.5204503744134853No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAA1124200.46300512069166255No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGG885530.3647081698328482No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTTA829700.3417144179308597No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAAT734610.3025513180139675No Hit
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC710450.292600950004796Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG698140.2875310398146925No Hit
TGGATAATGAATTATAATTAATATTATTAATTAATATTTATTATTAATAAT596760.24577738465037946No Hit
TGGAGTAATTTAGGTTGGTTTTTATTTATTTTAAATTTTTTTTTGTATGAA478970.19726522207586342No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC471470.1941763247220229TruSeq Adapter, Index 7 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCA460020.1894606080951598Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCGCGTATGC443370.18260325596963392TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG441320.1817589573595842No Hit
TGGTGGGGTTGAAGAAGGTGGTGTTGAGGTTGTGGTTTGTTAGTAGTATAG427490.17606303063910234No Hit
TGGTGGGGATAGTGATGATTATGGTAGTGGAGGTGAAATATGTTTGTGTGT424330.1747615752206842No Hit
TGGAAAAAAAGAATTATTTGGATATATAGGTATGGTTTGAGTTATGATATT402150.16562667611292664No Hit
GAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGCTC397680.1637856932900377Illumina Single End PCR Primer 1 (96% over 28bp)
TGGTTTTAGTGTTTTGTAATGTTATTGTGTGTATATTTTTTAGATGAAAAT392010.1614504868905343No Hit
TGGTTGTTAGGTGTTTAATGGGGTTTAGTAGGGTGGGGTTATTTTTGTTAA385010.15856751602694985No Hit
TGGGTTTATAATATTTGGGGGTAGTTAAAGTGAATTGTATTTGATATTTGG380560.15673477026367116No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG362860.1494449725086076No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG341080.14047481459305486No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA340370.1401823989768913No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC331140.13638099596676495No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG312460.1286875822907996No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG305060.1256398702350103No Hit
TGGATAGGTTGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTATGTTGATGA280210.11540532366928552No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG260110.10712707876099302No Hit
CGGGAGGTGAGGGGCGTTTTTGTTTGAGATCGGAAGAGCACACGTCTGAAC250970.10336274251911276Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTC246360.10146410027895214TruSeq Adapter, Index 7 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)