FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24280509
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2214200.9119248694498125No Hit
CAATCATATAACAATAAAAATAATTTAATTTAAACATCCAAGATCGGAAGA1246490.513370621678483No Hit
CAAACAATAAACCATAACCAATACTACCAATCAATACTCATCATTAATAAT934500.38487661028852405No Hit
CAAAATAATAAATTACAATATAAAAAATTATTCCAAAACCTAATAAAATAA842040.34679668371037853No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA812340.33456465018917025No Hit
CAAAAAAAAAAACCCCATTCTATACCAACACCTATTCTAATTTTTCAATCA745660.3071022934486258No Hit
CAAAATAATCCAAATCAATTTCTATCTACTTCAAATTCCTCCCTATACAAA724120.29823098024839595No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG666470.27448765592187546Illumina Single End PCR Primer 1 (100% over 32bp)
CAAATATCAAAAAAATACCTTAAATAACCTCTAAAACTCAAAAATAAAAAA606610.24983413650842326No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA584310.24064981504300426No Hit
CAAACTCTACCATCTTAACAAACCCTATTCTTAAATAAATATAAATATAAT489920.2017750122124705No Hit
GGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGA392730.16174702103650299Illumina Single End PCR Primer 1 (100% over 29bp)
CAAAACAAAAACAAATTAAACAATCTACCCTCCCTTAACAAAAAACTACTC387720.15968363760413753No Hit
CAAACCCATAACACTTAAAAATAACTAAAATAAACTATATCCAACATCTAA386780.1592964958024562No Hit
CAATAAAAATAACAATAATTATAATAACAAAAATAAAATATACTCATATAT385250.15866636074227275No Hit
CAACTCCAACATCTCACAATACTATCACATACATACCCCCCAAACAAAAAT383080.15777263977456155No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG363390.14966325458827903No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG328910.13546256382022304No Hit
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG325770.13416934546141515No Hit
CAAATCCATCATCCACAACCTTAACAATAAACAAAATATTCAAAAAATAAA318680.13124930782958463No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG317700.1308456919086828No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG314120.1293712582384496No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTAGACTGGAGTTCAGACGTGTGCTC292080.12029401854796373Illumina Single End PCR Primer 1 (96% over 30bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA279520.11512114511273219No Hit
CAACATCAAAATATTTAACTAACTCACCACACTCCACAAAAACAATATAAA249330.10268730363107298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)