FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28668975
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG1237830.4317663955547765No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1126560.39295440454358765TruSeq Adapter, Index 7 (100% over 51bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG763540.2663297170547604No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA662700.23115580518661724No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCC493170.17202219472443644TruSeq Adapter, Index 7 (98% over 51bp)
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC492210.17168733796726252No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG482010.16812948492228968No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTA394820.1377168175702131No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATAG366460.1278245908687004No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG346120.1207298133260781No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACAC342610.11950549330766097No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG332360.11593019980658534No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTA313470.10934119549094448No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)