FastQCFastQC Report
Sat 1 Apr 2023
EGAF00003471695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52370237
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG2437940.4655201388529137TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTATG1799900.3436875796456678TruSeq Adapter, Index 18 (97% over 40bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG1762030.3364563731113151No Hit
TGGATGTTTAAATTAAATTATTTTTATTGTTATATGATTGAGATCGGAAGA1744730.3331529700734408No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAA1494850.2854388457321665No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGG1141490.2179654065724392No Hit
CGGACGGGGCGGTTGGTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1123220.21447678382666094Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG1085460.20726658158908084No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTTA1085180.20721311610638693No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG992730.18955995940976933No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC975060.186185905555478No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG955570.1824643260636762No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAAT947030.18083362884151163No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA832540.1589719748642726No Hit
TGGATAATGAATTATAATTAATATTATTAATTAATATTTATTATTAATAAT774970.14797908972609766No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG753300.1438412432618932No Hit
CGGGCGGGGGGTTGATTTTTTTATTTTTTTTTTGAGATCGGAAGAGCACAC719810.13744638963539538No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTATGC685680.13092932919131148TruSeq Adapter, Index 18 (97% over 39bp)
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCCA682520.1303259330294801Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG637910.12180773594742372No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC633360.1209389218536475TruSeq Adapter, Index 18 (97% over 39bp)
TGGAGTAATTTAGGTTGGTTTTTATTTATTTTAAATTTTTTTTTGTATGAA618680.11813580297526628No Hit
CGGTTAAAACGGTGAAATTTCGTTTTTATTAAAAATATAAAAAATTAGTTG598030.11419272362658967No Hit
GAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACACGACGCTC558240.10659489663947865Illumina Single End PCR Primer 1 (96% over 28bp)
TGGTGGGGATAGTGATGATTATGGTAGTGGAGGTGAAATATGTTTGTGTGT552410.10548166891053022No Hit
TGGTGGGGTTGAAGAAGGTGGTGTTGAGGTTGTGGTTTGTTAGTAGTATAG545850.10422904903027268No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATAG530550.10130754229735489No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)