FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52370237
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2867140.5474750858965943No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA2025560.38677693973391797No Hit
CAATCATATAACAATAAAAATAATTTAATTTAAACATCCAAGATCGGAAGA1736690.33161774692751533No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA1460750.27892751373265695No Hit
CAAACAATAAACCATAACCAATACTACCAATCAATACTCATCATTAATAAT1210690.23117901872393665No Hit
CAAAATAATAAATTACAATATAAAAAATTATTCCAAAACCTAATAAAATAA1107230.21142352286853314No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1055230.20149421893966227Illumina Single End PCR Primer 1 (100% over 32bp)
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG1023110.19536096428205968No Hit
CAAAAAAAAAAACCCCATTCTATACCAACACCTATTCTAATTTTTCAATCA993490.18970508000565284No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG974240.1860293280704458No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG961730.18364056668294246No Hit
CAAAATAATCCAAATCAATTTCTATCTACTTCAAATTCCTCCCTATACAAA939030.17930604362168534No Hit
CAAATATCAAAAAAATACCTTAAATAACCTCTAAAACTCAAAAATAAAAAA792790.15138178580326073No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG757240.14459357898265765No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG719760.13743684222777147No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA703690.1343683054174454No Hit
CAAACTCTACCATCTTAACAAACCCTATTCTTAAATAAATATAAATATAAT637650.12175808942777938No Hit
GGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGA580410.11082821717992225Illumina Single End PCR Primer 1 (100% over 29bp)
CAACTAATTTTTTATATTTTTAATAAAAACGAAATTTCACCGTTTTAACCG575510.10989257123277865No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG568170.10849101179358801No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA566590.10818931371267233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)