FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24832614
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATAA1332990.5367900455425272No Hit
TGGATGTTAGAGGGGTGTTTTGGGTAATTTTTGGGATTTAGAAGTGAAAGG1077990.4341025072914193No Hit
TGGAGGAGGAGATTTTATTTTATATTAATATTTATTTTGATTTTTTGGTTA841760.33897357724804966No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAG822760.331322348907771No Hit
TGGGTTTTGTTATTTTAATAAATTTTGTTTTTGGGTGGGTGTGGGTATAAT728340.29329977101887056No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG672380.27076489007560783TruSeq Adapter, Index 27 (97% over 44bp)
TGGATAATGAATTATAATTAATATTATTAATTAATATTTATTATTAATAAT608610.2450849515882621No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAG512370.20632946656360865No Hit
TGGAGTAATTTAGGTTGGTTTTTATTTATTTTAAATTTTTTTTTGTATGAA412360.16605581675775252No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTA403060.16231074183330035No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCGCGTATG370280.14911035946517753TruSeq Adapter, Index 27 (97% over 45bp)
TGGTTGTTAGGTGTTTAATGGGGTTTAGTAGGGTGGGGTTATTTTTGTTAA332780.1340092508988381No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTTTTAGTTG319280.12857285181495592No Hit
TGGTTTTAGTGTTTTGTAATGTTATTGTGTGTATATTTTTTAGATGAAAAT317740.12795269962316494No Hit
CGGTCGTTTTTATTGGGAAGTGAGGAGTTTTTTTGTTTGAGATCGGAAGAG310200.12491637006075962No Hit
CGGGTAGAGGGGTTTTTTATTTTTTAGTAGGGGCGGTTGAGATCGGAAGAG292350.11772824238318205No Hit
CGGGAGGGAGGTGGGGGGGTTAGTTTTTCGTTTGAGATCGGAAGAGCACAC287550.11579530048669061No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGT286740.11546911654165767No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTT283100.11400330227015167No Hit
TGGGTTTATAATATTTGGGGGTAGTTAAAGTGAATTGTATTTGATATTTGG278050.1119696863165513No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCG276400.11130523753963237No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATAG269550.1085467683748477No Hit
TGGTGGGGTTGAAGAAGGTGGTGTTGAGGTTGTGGTTTGTTAGTAGTATAG249830.10060559875009535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)