FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32438785
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1425370.4394030170982052No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA973410.300075973868935No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG936850.2888055147564867No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG896420.27634203932113977No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG875380.2698559764183523No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG645760.19907034125969886No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG543200.16745386733812626No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA471150.14524280117149888No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAAT397130.12242443729011429No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA384780.11861726633719481No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTC383120.11810553323745017No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG325820.10044149310771042Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)