FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42077710
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1856850.4412906500852827No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG1289210.3063878713931913No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA1272650.3024522960018499No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG1236460.29385154277644865No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG1189140.28260568362679434No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG878680.2088231512598951No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG669130.1590224372951855No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG611010.14520989854248245Illumina Single End PCR Primer 1 (100% over 32bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA610980.14520276887691846No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAAT547410.130095007546751No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTC537170.1276614150342307No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA502430.11940526231109058No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)