FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003471714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39715813
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1486700.37433452514241616No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA1082950.2726747655902197No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG916820.2308450792635165No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG867920.2185326031220864No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG665940.16767628551378264No Hit
GAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG612040.15410486498161324No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG587630.14795869846602408No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG541630.13637641007122275No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG526760.13263230945316415Illumina Single End PCR Primer 1 (100% over 32bp)
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTC417700.10517221440235908No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAAT415120.10452259909673761No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)