FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29224791
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1167340.3994348496795067No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA805050.2754681804225734No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG753260.25774692452034986No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG725640.248296044272823No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG715720.2449016658493811No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG529260.1810996697974675No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG457370.15650069148484244No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG401040.13722595997350331Illumina Single End PCR Primer 1 (100% over 32bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA379450.12983839644909692No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAAT325250.1112924982081138No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTC315020.1077920454589393No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA312870.10705636868369735No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)