FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003471724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003471724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34465671
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAA1624970.47147493516084454No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAA1130640.32804816131390563No Hit
CAAACAAAAAAACTCCTCACTTCCCAATAAAAACGACCGAGATCGGAAGAG1038210.3012301719006138No Hit
CAAACGAAAAACTAACCCCCCCACCTCCCTCCCGAGATCGGAAGAGCGTCG1005140.2916351171575914No Hit
CAACCGCCCCTACTAAAAAATAAAAAACCCCTCTACCCGAGATCGGAAGAG1002450.2908546303944003No Hit
CAAAAAAAAAATAAAAAAATCAACCCCCCGCCCGAGATCGGAAGAGCGTCG727330.21103027415308406No Hit
CAACTAAAACGATAAAACCCCGTCTCTACTAAAAATACAAAAAATTAACCG557640.16179577644085327No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACA551670.16006361808536965No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACA447980.12997860973024433No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAAT432270.12542045097569696No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTC431860.12530149202666038No Hit
CAACCAACCGCCCCGTCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG381970.11082621893535745Illumina Single End PCR Primer 1 (100% over 32bp)
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACG381750.1107623873041671No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)